TRANSLATIONAL AND CLINICAL MEDICINE - Georgian Medical Journal

Introduction

Peripheral nerve injury is a common complication. The rate of peripheral nerve is assessed between 13 and 23 per100,000 persons per year injuries in developed countries. Angiogenesis, the formation of new blood vessels, is necessary in many physiological stages. In this experimental study, effects Chitosan/Poly ethylene glycol scaffold and Wharton jelly-derived mesenchymal stem cell increases angiogenesis in the Damaged sciatic nerve was investigated.

Methods & Materials

In this experimental study, male Wistar rats (n=42, 180-200g) were randomly divided into Control, Sham, Membrane, Cell and Mem+Cell groups. Regeneration of sciatic nerve was evaluated using histological analysis, angiogenesis (number of blood vessels and vessel area).

Results

At 8 weeks after surgery, the number of blood vessels measured in 10000 μm2 and vessel area increased significantly in experimental groups compared to control group (P<0.05). The number of blood vessels and vessel area increased significantly in Mem+Cell group compared to other experimental groups(P<0.05).

Conclusion

Chitosan/Poly ethylene glycol scaffold and Wharton jelly-derived mesenchymal stem cell increases angiogenesis in the Damaged sciatic nerve.

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